解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (1 ): 6-12.doi: 10.3969/j.issn.0529-1356.2013.01.002

• 神经生物学 • 上一篇    下一篇

小鼠Cajal-Retzius细胞发生及其细胞周期相关蛋白的表达

金海啸,符星,孔维芳,马战友,高文静,邓锦波*   

  1. 河南大学神经生物学研究所，河南 开封 475004
  • 收稿日期:2012-01-18 修回日期:2012-03-22 出版日期:2013-02-06 发布日期:2013-02-06
  • 通讯作者: 邓锦波 E-mail:jinbo_deng@henu.edu.cn
  • 基金资助:

    中国国家自然科学基金面上项目;国家973计划基金资助项目

Mouse Cajal-Retzius cells’histogenesis and expression of the cell cycle-related proteins

JIN Hai-xiao,FU Xing,KONG Wei-fang,MA Zhan-you, GAO Wen-jing, DENG Jin-bo*   

  1. Institute of Neurobiology, He’nan University, He’nan Kaifeng 475004, China
  • Received:2012-01-18 Revised:2012-03-22 Online:2013-02-06 Published:2013-02-06
  • Contact: DENG Jin-bo E-mail:jinbo_deng@henu.edu.cn

摘要：

目的 观察小鼠Cajal-Retzius（CR）细胞的发生以及多种细胞周期相关蛋白的表达情况。 方法 各日龄共计83只小鼠，应用免疫荧光法、溴脱氧尿嘧啶核苷（BrdU）等技术对胚胎和出生后小鼠进行实验。结果 1.CR细胞在E10和E11发生，它们主要分布在新皮质分子层和海马分子层，其密度随日龄的增长而逐渐降低。2.从E15到成年，细胞周期蛋白（cyclin）A2、cyclin E1和CDT1在CR细胞中持续表达，但始终未发现cyclin D1阳性的CR细胞。 结论 CR细胞对大脑发育有重要作用。CR细胞密度的减小趋势与新皮质发育的活跃程度相关。CR细胞已经退出了细胞周期而进入了G0期。如果CR细胞再次分裂，它们会因其轴突无法吸收并反向运输神经营养因子而凋亡。

关键词: 大脑皮质, 发育, Cajal-Retzius细胞, 免疫荧光技术, 小鼠

Abstract:

Objective To study Cajal-Retzius (CR) cells’ histogenesis and their expressions of cell cycle-related proteins in mouse. Methods Total 83 mice were used. Immunofluorescent labeling and BrdU assay were carried out at various embryonic and postnatal ages. Results 1. The birthday of CR cells was at about embryonic day 10 (E10) or 11 (E11). CR cells mainly distributed in the molecular layer of neocortex and hippocampus. Their number decreased with age increasing. 2.From E15 to adult, Cyclin A2, Cyclin E1, and CDT1 were expressed continuously in CR cells, but there was no any expression of Cyclin D1 in CR cells. Conclusion CR cells are important in the brain development. The decrease of CR cells with development is correlative with the activity of the neocortical development. CR cells in neocortex have been probably exited out cell cycle and steped in the G0 phase. If the CR cells split again, they will apoptosis because of their axons can not absorb and reversetransport the neurotrophic factors.

Key words: Cerebral cortex, development, Cajal-Retzius cells, immunocytochemistry, mouse